3-methyladenine (3MA) is a specific inhibitor of autophagy with activity against Vps34 and PI3Kg at IC50 values of 25 and 60 uM, respectively. [1] At concentrations of 5 mM, 3-methyladenine inhibits protein degradation in isolated rat hepatocytes by about 60% without any adverse effects on exogenous protein, protein synthesis, or intracellular ATP levels. [2]

3-methyladenine was shown in time-dependent studies to inhibit class I and class III PI3K in different manners when compared to wortmannin. [3] In an inflammation model setting, 3-methyladenine was found to enhance LPS-induced NF-kB activation and production of TNF-a, iNOS, COX-2, IL-1b, and IL-12. Through the inhibition of Akt by way of class I PI3K interference, 3-methyladenine can positively regulate p38, JNK, and p65, while negatively regulate TBK1 and IFN regulatory factor 3. [4]

3-methyladenine (3MA) is a specific inhibitor of autophagy with activity against Vps34 and PI3Kg at IC50 values of 25 and 60 uM, respectively. [1] At concentrations of 5 mM, 3-methyladenine inhibits protein degradation in isolated rat hepatocytes by about 60% without any adverse effects on exogenous protein, protein synthesis, or intracellular ATP levels. [2]

3-methyladenine was shown in time-dependent studies to inhibit class I and class III PI3K in different manners when compared to wortmannin. [3] In an inflammation model setting, 3-methyladenine was found to enhance LPS-induced NF-kB activation and production of TNF-a, iNOS, COX-2, IL-1b, and IL-12. Through the inhibition of Akt by way of class I PI3K interference, 3-methyladenine can positively regulate p38, JNK, and p65, while negatively regulate TBK1 and IFN regulatory factor 3. [4]