53AH is a selective Wnt pathway inhibitor. It is a cyclohexyl analog of IWR-1 with defined centers of chirality. In the Wnt assay, the 53AH has 5-fold greater potency in Wnt inhibition. Compared to IWR-1, 53AH is more potent at promoting stem cell differentiation into cardiomyocytes. The increase in Wnt pathway inhibition potency correlates with a 1.4-fold increase in cardiogenesis. 53AH possesses superior physicochemical properties compared to IWR-1 including improved water solubility and significantly increased chemical stability at pH = 7.4 and low cytotoxicity. Accordingly, 53AH is significantly more robust for long-term cell-based experiments and potency in cardiogenesis because of its superior chemical properties.

53AH is a selective Wnt pathway inhibitor. It is a cyclohexyl analog of IWR-1 with defined centers of chirality. In the Wnt assay, the 53AH has 5-fold greater potency in Wnt inhibition. Compared to IWR-1, 53AH is more potent at promoting stem cell differentiation into cardiomyocytes. The increase in Wnt pathway inhibition potency correlates with a 1.4-fold increase in cardiogenesis. 53AH possesses superior physicochemical properties compared to IWR-1 including improved water solubility and significantly increased chemical stability at pH = 7.4 and low cytotoxicity. Accordingly, 53AH is significantly more robust for long-term cell-based experiments and potency in cardiogenesis because of its superior chemical properties.