This kit extracts high-purity plasmid DNA from 1 - 4 ml of overnight cultured bacterial cultures, utilising an optimized alkaline lysis method, which improves the efficiency of nucleic acid release. A unique silica matrix membrane adsorption technology is emplyed to bind plasmids effectively while removing proteins, polysaccharides, and other impurities. A unique indicator-containing P2 solution provides visual confirmation of complete lysis and neutralization, ensuring extraction quality and facilitating visual operation. The yield of extracted plasmid DNA is high, requiring only 8 minutes, and the purified plasmid DNA can be directly used for restriction endonuclease digestion, PCR, sequencing, ligation, conversion, transfection of certain conventional cell lines, and other biological experiments.

This kit extracts high-purity plasmid DNA from 1 - 4 ml of overnight cultured bacterial cultures, utilising an optimized alkaline lysis method, which improves the efficiency of nucleic acid release. A unique silica matrix membrane adsorption technology is emplyed to bind plasmids effectively while removing proteins, polysaccharides, and other impurities. A unique indicator-containing P2 solution provides visual confirmation of complete lysis and neutralization, ensuring extraction quality and facilitating visual operation. The yield of extracted plasmid DNA is high, requiring only 8 minutes, and the purified plasmid DNA can be directly used for restriction endonuclease digestion, PCR, sequencing, ligation, conversion, transfection of certain conventional cell lines, and other biological experiments.