KU0063794 is an azaquinazoline-based [1], potent and highly selective inhibitor of mTORC1/2 with IC50 values of ~10 nM. [2] in a kinase panel at 1 uM KU0063794, none of the kinases, including the PI3K family, were inhibited, with the exception of MAPK kinase-1 which was decreased ~55%. [1] In HEK-293 cells in the presence of serum, KU0063794 inhibited S6K1 phosphorylation at Thr389 at concentrations as low as 30 nM. Consequently, inhibition was also observed at Thr229, along with ribosomal S6 protein. KU0063794 was also shown to inhibit phosphorylation of Akt at Thr308 at an IC50 of 86 nM. [3] In cell cycle studies, cell growth was inhibited and cells in the G1 phase were increased two-fold. [2]