EGFP mRNA expressed enhanced green fluorescent protein (enhanced green fuorescent protein, EGFP), which was initially isolated from jellyfish (Aequorea victoria) with a maximum emission wavelength of 509 nm, and the excited green fluorescence could be easily detected by fluorescence microscopy or flow cytometry. As a reporting mRNA, EGFP mRNA is ideal for monitoring and optimizing transfection efficiency and can be used as a positive control for mRNA transfection.

This mRNA contains high level of Cap1 structure at 5' terminal .Compared with the Cap 0 structure, the mRNA with the Cap 1 structure is more suitable for the mammalian system and has a higher transcription efficiency. Modified UTP and addition of poly(A) tails provide higher transcriptional efficiency and inhibit RNA-mediated innate immune activation while enhancing mRNA stability and longevity in vitro and in vivo.

EGFP mRNA expressed enhanced green fluorescent protein (enhanced green fuorescent protein, EGFP), which was initially isolated from jellyfish (Aequorea victoria) with a maximum emission wavelength of 509 nm, and the excited green fluorescence could be easily detected by fluorescence microscopy or flow cytometry. As a reporting mRNA, EGFP mRNA is ideal for monitoring and optimizing transfection efficiency and can be used as a positive control for mRNA transfection.

This mRNA contains high level of Cap1 structure at 5' terminal .Compared with the Cap 0 structure, the mRNA with the Cap 1 structure is more suitable for the mammalian system and has a higher transcription efficiency. Modified UTP and addition of poly(A) tails provide higher transcriptional efficiency and inhibit RNA-mediated innate immune activation while enhancing mRNA stability and longevity in vitro and in vivo.