Repro-nLS3i (also named LSB3i in reference 1) contains two cocktails of total five small molecule inhibitors. According to literature^1-3, the combination of these inhibitors significantly accelerated the process of deriving postmitotic neurons from human ESCs and iPSCs. Using this combination1, hESCs or hiPSCs rapidly differentiated into neurons with more than 75% efficiency within 10 days of differentiation. The resulting neurons present classical markers and functional properties of human nociceptors, including tetrodotoxin (TTX)-resistant, SCN10A-dependent sodium currents and response to nociceptive stimuli such as ATP and capsaicin1.

This differentiation process is rapid, efficient and can be easily scaled-up. Modulating cell fate of hPSC by Repro-nLS3i or similar strategies provides a method for basic stem cell research and drug discovery.

Repro-nLS3i (also named LSB3i in reference 1) contains two cocktails of total five small molecule inhibitors. According to literature^1-3, the combination of these inhibitors significantly accelerated the process of deriving postmitotic neurons from human ESCs and iPSCs. Using this combination1, hESCs or hiPSCs rapidly differentiated into neurons with more than 75% efficiency within 10 days of differentiation. The resulting neurons present classical markers and functional properties of human nociceptors, including tetrodotoxin (TTX)-resistant, SCN10A-dependent sodium currents and response to nociceptive stimuli such as ATP and capsaicin1.

This differentiation process is rapid, efficient and can be easily scaled-up. Modulating cell fate of hPSC by Repro-nLS3i or similar strategies provides a method for basic stem cell research and drug discovery.