Purmorphamine, a purine-based agonist of the Hedgehog signaling pathway, selectively induces osteogenesis in multipotent mesenchymal progenitor cells. In addition, purmorphamine regulates downstream targets such as Gli1 and Patched, which led to the discovery of its direct effect on Smoothened. [1, 3] Purmorphamine acts in contrast to known Hedgehog antagonists, such as cyclopamine and forskolin. In a luciferase-based assay, the EC50 of purmorphamine was found to be 0.5 uM. [1]

In an independent study on C3H10T1/2 cells, the EC50 of purmorphamine was found to be 1 uM, inducing a 50-fold increase in alkaline phosphatase (ALP). [2, 3]

Purmorphamine, a purine-based agonist of the Hedgehog signaling pathway, selectively induces osteogenesis in multipotent mesenchymal progenitor cells. In addition, purmorphamine regulates downstream targets such as Gli1 and Patched, which led to the discovery of its direct effect on Smoothened. [1, 3] Purmorphamine acts in contrast to known Hedgehog antagonists, such as cyclopamine and forskolin. In a luciferase-based assay, the EC50 of purmorphamine was found to be 0.5 uM. [1]

In an independent study on C3H10T1/2 cells, the EC50 of purmorphamine was found to be 1 uM, inducing a 50-fold increase in alkaline phosphatase (ALP). [2, 3]